Journal: Advanced Biotechnology
Article Title: Nanopore direct RNA sequencing for RNA modification analysis: workflow assessment and computational tool benchmarking
doi: 10.1007/s44307-025-00093-5
Figure Lengend Snippet: Workflow summaries of Sequencing methods with key procedures and devices (Wang, et al. ; Kchouk, et al. ; Tamang ; Sagar ). A Overall Diagram of Sanger First-generation Sequencing Method. B Four fundamental steps for the NGS framework comprising Extraction, Preparation, Sequencing, and Analysis. C-F Devices introduced by Oxford Nanopore Technology (ONT): MinION Products ( C ), Flongle adapter ( D ), PromethION ( E ), and GridION products ( F ). G Simplified procedures and elements of a representative TGS technology of ONT Nanopore Sequencing. Note: This Figure has been created in BioRender https://BioRender.com
Article Snippet: Third-generation Sequencing (TGS) technologies, notably Pacific Biosciences (PacBio) and Oxford Nanopore Technologies (ONT), have addressed several limitations by enabling single-molecule, long-read sequencing without amplification (Cuber, et al. ; McGinn and Gut ; Lin, et al. ; Amarasinghe, et al. ; PacBio ; Zhang, et al. , ).
Techniques: Sequencing, Extraction, Nanopore Sequencing